Issue Cover image

logo

Freshwater Crayfish 2(1): 211-225 (1975)

PEER REVIEWED    RESEARCH ARTICLE

Download: PDF (1.33 MB)

Microfluorometric determination of calcium in the hemolymph and other tissues of the crayfish

Adegboye JD and Hirsch PF  e-mail link

Published Online:

Abstract

Calcium (Ca) concentration in the hemolymph of the crayfish was determined by a fluorometric micro-methos which was precise, rapid, and applicable to solutions containing as little as 0.05?g of Ca. Hemolymph (5?l) was deproteinized in 2 ml 10% (W/V) trichloroacetic acid. Then 3 ml of cacein solution (1.2 mg calcein per 100 ml 0.9N KOH) was added to the hemolymph-acid mixture. The fluorescence of the Ca-calcein complex was measured. The method, which compared favorably with automated spectrophotometric analysis for Ca, was suitable for repeated sampling of hemolymph from the same crayfish.

Supplemental Documents

  • There are no supplementary documents for this article

CrossRef Logo

Cited By

Citations:

How to Cite

Adegboye JD and Hirsch PF. (1975). Microfluorometric determination of calcium in the hemolymph and other tissues of the crayfish. Freshwater Crayfish 2(1):211-225. doi: 10.5869/fc.1975.v2.211

 

 

Author Information

Authorship information for this paper are currently unavailable.

 

Publication History

   Manuscript Submitted:

   Manuscript Accepted:

   Published Online:

   Published in Print:

 

 

Funding Information

No specific funding statement is available for this article.

 

 



 

 

 

Member Login

Forgot Your Password?

Recover PW

Enter the e-mail address you used to
create your IAA account.
Return to Login
Back to Top